Green Tea Reduces Indomethacin-induced Damage in AGS Cells
Keywords:
AGS cells, Green tea extract, Indomethacin, MTT assay, ROSAbstract
Non-steroidal anti-inflammatory drugs (NSAIDs) disrupts mucosal defense through inhibition of cyclooxygenase (COX) and depletion of endogenous prostaglandins, thus exerts its adverse effects. Tea flavonoids; catechins and its derivatives, in particular, are known to prevent various systemic diseases like ulcer, cancer, atherosclerosis, hypertension, and diabetes that occur due to oxidative stress. Green tea is the most abundant source of catechins and its derivatives. In the present study, we aimed to determine the dose dependent toxicity of conventional NSAIDs, indomethacin on cultured AGS cells using MTT assay and morphological deformities by DAPI and acridine orange/ethidium bromide (AO/EtBr) fluorescence staining where aqueous extract of green tea (aGTE) provided significant protection. In the future, aGTE will be useful in designing and development of a novel protective supplement against NSAIDs that have reduced gastrointestinal toxicity while maintaining their essential therapeutic efficacy to inhibit pain and inflammation.
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